Phenotype Image Detail

Image

Caption

Flnb^tm1Vshn/Flnb^tm1Vshn (FlnB^-/-) mice display reduced Col10a1⁺/Col2a1⁺ ratio in the growth plates of the appendicular bones. (A) Double immunostaining for Collagen2 (Col2a1) and Collagen10 (Col10a1) on p7 and 2 week old radius of mutant mice. The arrows indicate the overlapping region of expression between Col2a1 and Col10a1 is increased in the mutant mice. (B) Quantitative analysis of the Col10a1⁺ length relative to Col2a1⁺ length. The ratio of Col10a1⁺ length relative to Col2a1⁺ length (hypertrophic to proliferative zone ratio), is decreased in mutant mice from P7 to 2 weeks age (33.8% vs 45.3% and 71.9% vs 89.6% in mutant and wild-type (WT), respectively). The ratio at P1 age does not show significant change. (C) Quantitative analysis of the relative thickness of the Col10a1⁺ hypertrophic zone (Col10a1⁺ /whole growth plate) is increased at this age. **=p<0.01, ***= p <0.001 by Student's t-test. Scale bars= 200 um.

Copyright

This image is from Hu J, PLoS One 2014;9(2):e89352, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:213355

Associated Alleles

Symbol	Name
Flnb^tm1Vshn	filamin, beta; targeted mutation 1, Volney Sheen

Associated Genotypes

Allelic Composition	Genetic Background
Flnb^tm1Vshn/Flnb^tm1Vshn	involves: 129S6/SvEvTac * C57BL/6