Phenotype Image Detail

Image

Caption

The number of meiotic double stranded breaks (DSBs) is reduced in Spo11^tm1.1Gvpc/Spo11^tm1.1Gvpc (Spo11^Gal/Gal) and Spo11^tm1.1Gvpc/Spo11^tm3Rdco (Spo11^Gal/-) males. Chromosomal spreads from earlier (zygotene) and later (pachytene) stage spermatocytes were immunostained with antibodies to the DMC1 protein (red) to indicate the appearance and repair of DSBs in wild-type (WT) mice and mice carrying one or two mutant alleles. Chromosome cores were stained with antibodies to the axial element component SCP3 (green), and progression of homologous synapsis was monitored with antibodies to the component of the central element of the synaptonemal complex SCP1 (white).

Copyright

This image is from Smagulova F, BMC Genomics 2013;14():493, an open-access article, licensee BioMed Central Ltd. J:202231

Associated Alleles

Symbol	Name
Spo11^tm1.1Gvpc	SPO11 initiator of meiotic double stranded breaks; targeted mutation 1.1, Galina Petukhova
Spo11^tm3Rdco	SPO11 initiator of meiotic double stranded breaks; targeted mutation 3, R Daniel Camerini-Otero

Associated Genotypes

Allelic Composition	Genetic Background
Spo11^tm1.1Gvpc/Spo11^tm1.1Gvpc	involves: C57BL/6
Spo11^tm1.1Gvpc/Spo11^tm3Rdco	involves: C57BL/6