Phenotype Image Detail

Image

Caption

Abnormal megakaryocytopoiesis. In hematoxylin-eosinstaining, the morphology of megakaryocyte in Myh9^tm1.1Dash/Myh9⁺ (R702C+/-) mice was not different from that of wild-type (C57BL/6j; WT) mice (A) (B). There were no significant differences in the distribution of NMMHCIIA between heterozygous mutant mice and WT mice by immunostaining (C) (D). Proplatelet formation was explored by cultured fetal liver cells. In WT mice, the proplatelet shaft extends from the cell spindle, leading to the formation of proplatelet beads. Heterozygous mutant mice had shorter and thicker shafts when compared with WT mice, and the proplatelet beads were fewer and larger than those of WT mice. Megakaryocytes were stained with May Grunwald Giemsa (MGG) (E) (F) or were observed using CD41 immunofluorescence (G) (H).

Copyright

This image is from Suzuki N, PLoS One 2013;8(8):e71187, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:204923

Associated Alleles

Symbol	Name
Myh9^tm1.1Dash	myosin, heavy polypeptide 9, non-muscle; targeted mutation 1.1, Tadashi Matsushita

Associated Genotypes

Allelic Composition	Genetic Background
Myh9^tm1.1Dash/Myh9^+	involves: 129S2/SvPas * C57BL/6 * C57BL/6J * CBA