Phenotype Image Detail

Image

Caption

Klf5's heterozygous deletion (Klf5^tm1.1Jtd/Klf5⁺ Tg(Pbsn-cre)4Prb/0) promotes cell proliferation and induced hyperplasia while its homozygous deletion (Klf5^tm1.1Jtd/Klf5^tm1.1Jtd Tg(Pbsn-cre)4Prb/0) causes apoptosis in the prostate. Shown in the upper panels is the IHC staining of Ki67 in 1-year-old dorsal prostates, and the ratio of Ki67-positive cells is significantly higher in the prostate with Klf5 heterozygous deletion (Klf5+/-) than those with wild-type Klf5 or homozygous deletion (Klf5-/-) of Klf5 (upper right panel). The lower panels show apoptotic cells (indicated by arrows) as detected by TUNEL assay in the anterior prostates from 1.5-year-old mice, and the ratio of apoptotic cells in the homozygous deletion group is about double that in the wild-type or hemizygous deletion group (lower right panel). Three mice per genotype were counted.

Copyright

This image is from Xing C, PLoS One 2013;8(6):e65538, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:206639

Associated Alleles

Symbol	Name
Klf5^tm1.1Jtd	Kruppel-like transcription factor 5; targeted mutation 1.1, Jin-Tang Dong
Tg(Pbsn-cre)4Prb	transgene insertion 4, Pradip Roy-Burman

Associated Genotypes

Allelic Composition	Genetic Background
Klf5^tm1.1Jtd/Klf5^tm1.1Jtd Tg(Pbsn-cre)4Prb/0	involves: C57BL/6 * DBA/2
Klf5^tm1.1Jtd/Klf5^+ Tg(Pbsn-cre)4Prb/0	involves: C57BL/6 * DBA/2