Phenotype Image Detail

Image

Caption

Altered cell adhesion properties in Tbx1 mutants. (A) Paintfilling of inner ears from Gt(ROSA)26Sor^{tm1(Tbx1/GFP)Bem}/Gt(ROSA)26Sor⁺ Tg(Pax2-cre)1Akg/0 (Pax2-Cre;Tbx1-GFP^flox/+) mutants at progressively earlier stages of development reveals a delay in semicircular canal (SCC) formation. At E12.25, fusion plates (arrows, FP) are visible in the controls, but do not appear to have formed in the mutants. Histological analysis confirms defects in the fusion plates (FP, red arrows). In controls, the vestibular epithelia can be seen to have separated from the mesenchyme (asterisks). In Pax2-Cre;Tbx1-GFP^flox/+ mutants, there is no separation of the epithelium from surrounding tissue. (B) Immunofluorescence with anti-laminin (red). Arrows indicate the span of the fusion plate epithelia. There is more intact laminin in the Pax2-Cre;Tbx1-GFP^flox/+ mutants. RNA in situ hybridization to netrin-1 on sections. Netrin-1 expression is reduced in the fusion plates of Pax2-Cre;Tbx1-GFP^flox/+ mutants. Anterior canal (AC, lateral canal (LC).

Copyright

This image is from Freyer L, BMC Dev Biol 2013 Aug 23;13(1):33, an open-access article, licensee BioMed Central Ltd. J:204435

Associated Alleles

Symbol	Name
Gt(ROSA)26Sor^tm1(Tbx1/GFP)Bem	gene trap ROSA 26, Philippe Soriano; targeted mutation 1, Bernice E Morrow
Tg(Pax2-cre)1Akg	transgene insertion 1, Andrew K Groves

Associated Genotypes

Allelic Composition	Genetic Background
Gt(ROSA)26Sor^tm1(Tbx1/GFP)Bem/Gt(ROSA)26Sor^+ Tg(Pax2-cre)1Akg/0	involves: 129 * C57BL/6 * SJL