Phenotype Image Detail

Image

Caption

Ultrastructure of spermatozoa from cauda, caput and testis from wild-type (WT, +/+) littermates and Del(8Defb1-Defb13)1Jrdo/Del(8Defb1-Defb13)1Jrdo (-/-) male mice reveals a defect in microtubule structure. A: Transmission Electron Microscopy (TEM) of sperm from cauda, caput and testis from mutant and WT littermates. Top and middle horizontal panels show overviews of cross section of sperm from cauda of WT and mutant mice respectively at various levels of the tail (a). Higher magnification of cross section of principal piece (b-d) and mid-piece (e-f) of sperm tails. Upper panel (f) shows normal axoneme (9+2 microtubules, MT), mitochondrial sheath (M), outer dense fibres (ODF) in WT mouse sperm. Middle panel (f) shows clear disruption and disintegration of the MT (arrowhead) in sperm from the mutant. Middle panel (b) shows example of additional microtubules (*) other than the classical 9+2 arrangement. Bottom panel shows TEM of caput epididymal (a, b) and testis (c, d) sperm from WT and mutant mice. No obvious microtubule disruptions were observed in sperm within the caput or testis of the mutant mice. Bars: 500 nm and 100 nm as labelled (a-f). B: Fluorescent intensity of total intracellular calcium of WT littermates and mutant sperm using Fluo3 AM ester assay. The calcium levels were measured using Fluo-3 AM ester calcium fluorescent indicator at 5 uM concentration incubated with spermatozoa at 20 million/ml for 30 mins at 37 degrees C, samples were washed and loaded onto 96-well plate in duplicates at 100 ml/well, and the plates were read by BMG Labtech FluoSTAR Omega fluorescent reader (mean +/- SD; n = 3).*, p<0.002. Values given are after subtracting the background levels of the DMSO controls.

Copyright

This image is from Zhou YS, PLoS Genet 2013 Oct;9(10):e1003826, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:204187

Associated Alleles

Symbol	Name
Del(8Defb1-Defb13)1Jrdo	deletion, Chr 8, Julia R Dorin 1

Associated Genotypes

Allelic Composition	Genetic Background
Del(8Defb1-Defb13)1Jrdo/Del(8Defb1-Defb13)1Jrdo	involves: 129P2/OlaHsd * C57BL/6N