Phenotype Image Detail

Image

Caption

Abnormal heart development and coronary vasculogenesis after early cardiomyocyte-restricted Zfpm2 (Fog2) inactivation in Zfpm2^tm1Sho/Zfpm2^tm2Sho Nkx2-5^tm1(cre)Rjs/Nkx2-5⁺ (Fog2^NK) mice. (A and B) Control and littermate mutant embryos at E13.5. (C-H) Representative H&E staining of mutant (right panels) and littermate control (left panels) hearts at different levels. RA, right atrium; LA, left atrium; RV, right ventricle; A, aorta. The asterisk indicates atrioventricular (AV) cushion and the arrow indicates ventricular septal defect (VSD). (I-K) PECAM whole-mount staining of mutant and control hearts at E13.5. The coronary endothelial plexus coverage (ratio of region within black dotted line to total projected ventricular area) was significantly decreased in mutants. (*P < 0.05; n = 4-5 per group as indicated.) (L) Section of X-gal stained Tie2-Lz hearts showed decreased coronary vessels in the compact myocardial layer of mutant compared with littermate control. Scale bar: 500 um. Ventricles in the boxed regions are magnified (right panels).

Copyright

This image is from Zhou B, J Clin Invest 2009 Jun;119(6):1462-76 and is displayed with the permission of the American Society for Clinical Investigation who owns the Copyright. J:150452

Associated Alleles

Symbol	Name
Nkx2-5^tm1(cre)Rjs	NK2 homeobox 5; targeted mutation 1, Robert J Schwartz
Zfpm2^tm1Sho	zinc finger protein, multitype 2; targeted mutation 1, Stuart Orkin
Zfpm2^tm2Sho	zinc finger protein, multitype 2; targeted mutation 2, Stuart Orkin

Associated Genotypes

Allelic Composition	Genetic Background
Zfpm2^tm1Sho/Zfpm2^tm2Sho Nkx2-5^tm1(cre)Rjs/Nkx2-5^+	involves: 129S1/Sv * 129S7/SvEvBrd