Phenotype Image Detail

Image

Caption

Defective myocardial perfusion and coronary vasculature in adult Zfpm2^tm1Sho/Zfpm2^tm2Sho Tg(Myh6-cre)2182Mds/0 (Fog2^MC) heart. (A-F) Cleared, Microfil-injected control and mutant hearts. Microvascular filling by Microfil was markedly reduced in mutant hearts compared with control. (G) Perfusion labeling of endothelial cells by injected Bs-1 lectin. Myocardial labeling was reduced in the mutant. (H) Assessment of myocardial perfusion by myocardial ^99mTc-2 methoxy isobutyl isonitrile (MIBI) uptake. Uptake is expressed as a fraction of the injected dose, normalized to heart weight. (I and J) Representative figure of hypoxia in mutant myocardium. Hypoxia was detected as immunoreactivity to hypoxyprobe (hypoxy). Staining was greater in mutant myocardium, including cardiomyocytes (arrowheads). Boxed regions are magnified in center panels. Transmission" indicates phase-contrast images. (K) Decreased capillary density in mutant myocardium. PECAM+ vessels in histological sections were quantitated. (L) Decreased smooth muscle-positive vessels in mutant myocardium. (M) Increased endothelial cell apoptosis in mutant myocardium. The boxed apoptotic endothelial cell is magnified (right panel). Apoptosis was quantitated by TUNEL staining of PECAM+ coronary endothelial cells (white arrow). For quantitation in H and K-M, n = 3-4 for each group. *P < 0.05. Scale bar: 500 um (A, B, D, and E); 250 um (C and F); 50 um (G and I-M).

Copyright

This image is from Zhou B, J Clin Invest 2009 Jun;119(6):1462-76 and is displayed with the permission of the American Society for Clinical Investigation who owns the Copyright. J:150452

Associated Alleles

Symbol	Name
Tg(Myh6-cre)2182Mds	transgene insertion 2182, Michael D Schneider
Zfpm2^tm1Sho	zinc finger protein, multitype 2; targeted mutation 1, Stuart Orkin
Zfpm2^tm2Sho	zinc finger protein, multitype 2; targeted mutation 2, Stuart Orkin

Associated Genotypes

Allelic Composition	Genetic Background
Zfpm2^tm1Sho/Zfpm2^tm2Sho Tg(Myh6-cre)2182Mds/0	involves: 129S1/Sv * 129S7/SvEvBrd