Phenotype Image Detail

Image

Caption

Evaluation of resultant cardiovascular phenotypes in Gt(ROSA)26Sor^tm1(DTA)Jpmb/Gt(ROSA)26Sor⁺ Tg(Smad7-cre)1Sjc/0 embryos. (a) E10.5 Gt(ROSA)26Sor^tm1(DTA)Jpmb/Gt(ROSA)26Sor⁺ Tg(Smad7-cre)1Sjc/0 (mutant, left) and Gt(ROSA)26Sor^tm1(DTA)Jpmb/Gt(ROSA)26Sor⁺ only (control, right) embryo littermates. (b,c) Higher power images of ablated mutant (b) and normal control (c) cardiovascular and branchial arch regions of embryos shown in a. Note that the atrioventricular (AV) cushion region (*) is largely absent in mutant (b) hearts and that the arches are smaller in size (arrows). (d,e) High power images of the mutant (d) and normal control (e) yolk sacs, note the lack of vasculature and blood in mutant. (f) Counterstaining with alpha-SMA (that detects both smooth and cardiac muscle lineages at this stage of in utero development), reveals that the myocardium is largely unaffected via Smad7^Cre-mediated cell ablation but that the overall size of the heart is decreased, when compared to unaffected littermate photographed at same magnification (g). Also note that while the endocardial cushions are hypoplastic, the mutant endothelium remains intact (arrow, g). (h,i) alpha-SMA staining also reveals that whilst the control littermate has two distinct alpha-SMA-positive paired dorsal aorta (i), and the ablated mutant vessels have hemorrhaged and that there is fragmented alpha-SMA-stained smooth muscle cells within the mutant (h). Sections f-i were lightly counterstained with methyl green. Abbreviation: a = atria.

Copyright

This image is from Snider P, Genesis 2009 Jul;47(7):469-75, and is displayed with the permission of Wiley-Blackwell, who owns the Copyright. J:150864

Associated Alleles

Symbol	Name
Gt(ROSA)26Sor^tm1(DTA)Jpmb	gene trap ROSA 26, Philippe Soriano; targeted mutation 1, Juan Pedro Martinez-Barbera
Tg(Smad7-cre)1Sjc	transgene insertion 1, Simon J Conway

Associated Genotypes

Allelic Composition	Genetic Background
Gt(ROSA)26Sor^tm1(DTA)Jpmb/Gt(ROSA)26Sor^+ Tg(Smad7-cre)1Sjc/0	involves: 129S/Sv * C3HeB/FeJ