Phenotype Image Detail

Image

Caption

Differentiation in Apc^tm2Rfo/Apc⁺ mice. HE staining of mammary tumors (A-B) and pulmonary metastases (C) from the mutant mice shows typical mammary glandular architecture and squamous differentiation. (D-F) Luminal epithelial differentiation as shown by cytokeratin 8 (Ck8) IHC staining. (G-I) Myoepithelial differentiation revealed by IHC staining with the Sma antibody. (J-L) IHC analysis with antibodies directed against cytokeratin 14 (Ck14) confirm the presence of squamous differentiation (hair follicle and skin cellular types). (M-O) Beta-catenin IHC analysis shows heterogeneous subcellular localization and intracellular accumulation with fewer cells characterized by positive nuclear staining. The results shown in this figure were confirmed in 12 independent primary tumors.

Copyright

This image is from Gaspar C, PLoS Genet 2009 Jul;5(7):e1000547, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:151494

Associated Alleles

Symbol	Name
Apc^tm2Rfo	APC, WNT signaling pathway regulator; targeted mutation 2, Riccardo Fodde

Associated Genotypes

Allelic Composition	Genetic Background
Apc^tm2Rfo/Apc^+	B6.129P2-Apc^tm2Rfo
Apc^tm2Rfo/Apc^+	(C57BL/6J x 129P2/OlaHsd)F1
Apc^tm2Rfo/Apc^+	129P2/OlaHsd-Apc^tm2Rfo