Phenotype Image Detail

Image

Caption

Ablation of SPP1 leads to changes in intramuscular inflammatory cells. Infiltrating leukocytes were isolated from Dmd^mdx/Y Spp1^tm1Blh/Spp1^tm1Blh or Dmd^mdx/Dmd^mdx Spp1^tm1Blh/Spp1^tm1Blh (DMM) and Dmd^mdx/Y or Dmd^mdx/Dmd^mdx (mdx) mice and characterized by flow cytometry. Each experiment was performed on leukocytes isolated from muscles of 4 grouped 4-week-old mice, which were stained for cell surface markers and analyzed by flow cytometry. Cross sections of 6-month-old mdx and DMM diaphragm muscles immunostained for Ly6G, a neutrophil marker (red) and counterstained for hematoxylin (blue). A specific reduction in neutrophils but not macrophages is seen in DMM muscles.

Copyright

This image is from Vetrone SA, J Clin Invest 2009 Jun;119(6):1583-94 and is displayed with the permission of the American Society for Clinical Investigation who owns the Copyright. J:150572

Associated Alleles

Symbol	Name
Dmd^mdx	dystrophin, muscular dystrophy; X linked muscular dystrophy
Spp1^tm1Blh	secreted phosphoprotein 1; targeted mutation 1, Brigid L Hogan

Associated Genotypes

Allelic Composition	Genetic Background
Dmd^mdx/Y Spp1^tm1Blh/Spp1^tm1Blh	involves: 129S6/SvEvTac * C57BL/6 * C57BL/10 * C57BL/10ScSn
Dmd^mdx/Dmd^mdx Spp1^tm1Blh/Spp1^tm1Blh	involves: 129S6/SvEvTac * C57BL/6 * C57BL/10 * C57BL/10ScSn