Phenotype Image Detail

Image

Caption

Tibial epiphyseal growth plate abnormalities in n-TUtca2^tm1Dhat/n-TUtca2^tm1Dhat Tg(Col2a1-cre)1Bhr/0 mice. Relative sizes and morphological differences between the tibiae of (A) control and (B) mutant mice (hematoxylin and eosin stained sections) are shown. Note smaller bone size, and the disproportionate widening of the epiphyseal growth plate in the mutant mice. Higher magnification views of representative growth plate sections from control (C) and mutant (D) mice; there was widening of both the proliferative (white two-headed arrows) and hypertrophic (black two-headed arrows) zones in the mutant mice. BrdU+ nuclear staining revealed an increase in the number of mitoses within the tibial growth plate proliferative zones of control (E) as compared to mutant (F) mice (arrows indicate examples of BrdU+ cells). Scale bars were as follows: 450 um (A and B), 55 um (C and D) and 27 um (E and F). (G) Numbers of BrdU+ nuclei (n = 14 mutant and n = 7 control tibiae), and TUNEL positive apoptotic cells (n = 3 mutant and n = 5 control tibiae) present in the epiphyseal growth plates. Data are shown as mean +/- SEM.

Copyright

This image is from Downey CM, PLoS Genet 2009 Aug;5(8):e1000616, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:152152

Associated Alleles

Symbol	Name
n-TUtca2^tm1Dhat	nuclear encoded tRNA selenocysteine 2 (anticodon TCA); targeted mutation 1, Dolph L Hatfield
Tg(Col2a1-cre)1Bhr	transgene insertion 1, Richard R Behringer

Associated Genotypes

Allelic Composition	Genetic Background
n-TUtca2^tm1Dhat/n-TUtca2^tm1Dhat Tg(Col2a1-cre)1Bhr/0	B6.Cg-n-TUtca2^tm1Dhat Tg(Col2a1-cre)1Bhr