Phenotype Image Detail

Image

Caption

Auricular chondronecrosis and hypoplasia in n-TUtca2^tm1Dhat/n-TUtca2^tm1Dhat Tg(Col2a1-cre)1Bhr/0 mice. (A) Hematoxylin and eosin staining of ear cartilage from a control mouse, compared with (B) the mutant auricular cartilage that shows a wide band of necrotic chondrocytes. Detection of TUNEL positive cells (stained brown, with methyl-green counterstain) in control (C) and mutant (D) auricular cartilage. Note abundance of necrotic cells with scattered TUNEL positive cells in (D). Evidence of apparent regenerative activity of cartilage above and below the band of necrotic cartilage, but not in the control (E), is shown (F). Detection of cell proliferation using anti-PCNA antibody immunostaining on control (E) and mutant (F) cartilage; haematoxylin counter stain. Scale bars are as follows: 55 um (A,B,E,F), 27 um (C,D).

Copyright

This image is from Downey CM, PLoS Genet 2009 Aug;5(8):e1000616, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:152152

Associated Alleles

Symbol	Name
n-TUtca2^tm1Dhat	nuclear encoded tRNA selenocysteine 2 (anticodon TCA); targeted mutation 1, Dolph L Hatfield
Tg(Col2a1-cre)1Bhr	transgene insertion 1, Richard R Behringer

Associated Genotypes

Allelic Composition	Genetic Background
n-TUtca2^tm1Dhat/n-TUtca2^tm1Dhat Tg(Col2a1-cre)1Bhr/0	B6.Cg-n-TUtca2^tm1Dhat Tg(Col2a1-cre)1Bhr