Phenotype Image Detail

Image

Caption

Tracheomalacia and airway lumen narrowing in n-TUtca2^tm1Dhat/n-TUtca2^tm1Dhat Tg(Col2a1-cre)1Bhr/0 mice. (A) Triple-stained section of a trachea from a representative control mouse, showing normal cartilage ring cross-sections with abundant cells and glycosaminoglycan deposition in the extracellular matrix (indicated by red staining); the connective tissue is stained green. (B) Tracheal rings from a representative mutant mouse were reduced in size, and showed a dramatic loss of red staining as well as chondrocyte depletion; dystrophic calcification was also present in some cartilage rings, for example, the dark staining region in uppermost ring on the right side of the trachea in (B). The lumen of the mutant tracheas was also narrowed. TUNEL positive cells were not evident in the control tracheal cartilages (C), however, scattered TUNEL positive chondrocytes were present within areas of chondronecrosis within mutant tracheal rings (D). Methyl-green counterstaining, used to indicate positions of cell nuclei, also revealed loss of extracellular matrix staining in the mutant rings (D) consistent with chondrocyte dysfunction or death. Scale bars are 450 um (A and B), and 27 um (C and D).

Copyright

This image is from Downey CM, PLoS Genet 2009 Aug;5(8):e1000616, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:152152

Associated Alleles

Symbol	Name
n-TUtca2^tm1Dhat	nuclear encoded tRNA selenocysteine 2 (anticodon TCA); targeted mutation 1, Dolph L Hatfield
Tg(Col2a1-cre)1Bhr	transgene insertion 1, Richard R Behringer

Associated Genotypes

Allelic Composition	Genetic Background
n-TUtca2^tm1Dhat/n-TUtca2^tm1Dhat Tg(Col2a1-cre)1Bhr/0	B6.Cg-n-TUtca2^tm1Dhat Tg(Col2a1-cre)1Bhr