Phenotype Image Detail

Image

Caption

Homozygous deletion of Trim33 (Tif1gamma) cooperates with activated Kras^G12D mutation to induce cystic tumors of the pancreas in Kras^tm4Tyj/Kras^tm4Tyj Trim33^tm1Los/Trim33^tm1Los Tg(Pdx1-cre)89.1Dam/0 (Pdx1-cre;LSL-Kras^G12D;Tif1gamma^lox/lox) mice. Homozgyous deletion of Cdkn2a cooperates with activated Kras^G12D mutation to induce solid tumors in Kras^tm4Tyj/Kras^tm4Tyj Cdkn2a^tm4Rdp/Cdkn2a^tm4Rdp Tg(Pdx1-cre)89.1Dam/0 (Pdx1-Cre;LSL-Kras^G12D;Ink4A/Arf^lox/lox). Magnetic Resonance Imaging (MRI) (A-C). Positron Emission Tomography (PET) (D-F). Gross anatomy of the pancreas after dissection (G-I). Hematoxylin Phloxine Saffron (HPS) staining of pancreas sections (J-L). Acinar tissue (stars), scattered endocrine islets (arrow) and exocrine ducts (arrowhead). When notified, pancreas is circled in red dashed lines. Pdx1-cre;LSL-Kras^G12D;Tif1gamma^lox/lox mice used in this experiment were 154 days old and representative of the 4 mice we analyzed at the age of 18-27 weeks.

Copyright

This image is from Vincent DF, PLoS Genet 2009 Jul;5(7):e1000575, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:151781

Associated Alleles

Symbol	Name
Cdkn2a^tm4Rdp	cyclin dependent kinase inhibitor 2A; targeted mutation 4, Ronald DePinho
Kras^tm4Tyj	Kirsten rat sarcoma viral oncogene homolog; targeted mutation 4, Tyler Jacks
Tg(Pdx1-cre)89.1Dam	transgene insertion 89.1, Douglas A Melton
Trim33^tm1Los	tripartite motif-containing 33; targeted mutation 1, Regine Losson

Associated Genotypes

Allelic Composition	Genetic Background
Kras^tm4Tyj/Kras^tm4Tyj Trim33^tm1Los/Trim33^tm1Los Tg(Pdx1-cre)89.1Dam/0	involves: 129S2/SvPas * 129S4/SvJae * C57BL/6 * DBA/2
Cdkn2a^tm4Rdp/Cdkn2a^tm4Rdp Kras^tm4Tyj/Kras^tm4Tyj Tg(Pdx1-cre)89.1Dam/0	involves: 129S4/SvJae * C57BL/6 * DBA/2