Phenotype Image Detail

Image

Caption

Morphology of kidneys in wild-type (wt), Fgfrl1^tm2.1True/Fgfrl1⁺ (wt/ki) and Fgfrl1^tm2.1True/Fgfrl1^tm2.1True (ki/ki) mice. A) Kidneys from WT and mutant mice did not reveal any alterations in overall appearance two months after birth. Likewise, no difference was observed on paraffin sections stained with H&E. Bar = 100 um. B) Example of a thin section stained with H&E to demonstrate the strategy used to determine the number of glomeruli in kidney samples of E17.5. The number of glomeruli within each rectangle was counted under higher power magnification. Subsequently, the number of glomeruli per mm² was calculated for each sample. C) Statistical analysis of the number of glomeruli in WT, heterozygous and homozygous kidneys at E17.5. Numbers are given in relation to the numbers of WT kidneys that were arbitrarily set to 100%. Homozygous Fgfrl1^tm2.1True/Fgfrl1^tm2.1True, heterozygous Fgfrl1^tm2.1True/Fgfrl1⁺ and heterozygous Fgfrl1^tm1True/Fgfrl1⁺ mice showed a slight, but significant reduction in the total number of glomeruli (* p < 0.05 Student's t-test). The numbers of individual kidneys, which were analyzed in this fashion, were: WT (for knock-in littermates) 7, WT (for knock-out littermates) 5, heterozygous knock-in 17, heterozygous knock-out 7, homozygous knock-in 8. Homozygous knock-out animals do not develop any metanephric kidneys. Note that number of kidneys equals number of animals since only one kidney per animal was used for analysis.

Copyright

This image is from Bluteau G, PLoS One 2014;9(8):e105210, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:218981

Associated Alleles

Symbol	Name
Fgfrl1^tm2.1True	fibroblast growth factor receptor-like 1; targeted mutation 2.1, Beat Trueb

Associated Genotypes

Allelic Composition	Genetic Background
Fgfrl1^tm2.1True/Fgfrl1^tm2.1True	B6.Cg-Fgfrl1^tm2.1True
Fgfrl1^tm2.1True/Fgfrl1^+	B6.Cg-Fgfrl1^tm2.1True