Phenotype Image Detail

Image

Caption

Degeneration of Purkinje cells and cerebellar atrophy in Camta1^tm1.1Eno/Camta1^tm1.1Eno Tg(Nes-cre)1Kln/0 (CAMTA1-nKO) mice. (A) Gross morphology of brains from adult wild-type (WT) and mutant mice. (B) H&E stain of sagittal sections of brain from 3-week-old (Upper) and adult (Lower) WT and mutant mice. (Scale bar, 400 um.) (C) Calbindin-D28K immunohistochemical staining (red) of Purkinje cells in the cerebellum of 6-month-old mutant and littermate control mice. Purkinje cells are uniformly organized in the cerebellum of the control mouse but are reduced and disarrayed in the cerebellum of the mutant mouse. Nuclei are stained with DAPI (blue). gl, granular layer. (Scale bar, 100 um.) (D) Quantification of the size of calbindin-positive Purkinje cells in the cerebellum (n = 3). (E and F) H&E staining of sagittal sections of the brain from mutant and littermate control mice at age 2-3 weeks (E) and 3 months (F). White arrows point to Purkinje cells. (Scale bar, 400 um Upper, 40 um Lower.) n = 6; *P < 0.05. gl, granular layer; ml, molecular layer. (G and H) Quantification of the Purkinje cells of mutant and littermate control mice at age 2-3 weeks (G) and 3 months (H). Purkinje cell number is not reduced in mutant mice at age 2-3 weeks but is decreased at age 3 months along with reductions in the width of the molecular layer and granular layer. Data are presented as mean SD.

Copyright

This image is from Long C, Proc Natl Acad Sci U S A 2014 Aug 5;111(31):11521-6. Copyright 2014 National Academy of Sciences, U.S.A. J:212232

Associated Alleles

Symbol	Name
Camta1^tm1.1Eno	calmodulin binding transcription activator 1; targeted mutation 1.1, Eric N Olson
Tg(Nes-cre)1Kln	transgene insertion 1, Rudiger Klein

Associated Genotypes

Allelic Composition	Genetic Background
Camta1^tm1.1Eno/Camta1^tm1.1Eno Tg(Nes-cre)1Kln/0	involves: C57BL/6 * SJL