Phenotype Image Detail

Image

Caption

Loss and apoptosis of primordial germ cells early after specification in Mad2l2^tm1.1Mpz/Mad2l2^tm1.1Mpz mice. (A) Alkaline phosphatase-positive wild-type (+/+) or Mad2l2^tm1.1Mpz/Mad2l2^tm1.1Mpz (-/-) primordial germ cells (PGCs) were detected in early head fold and late head fold stages. From E8.5 to E9.5, primordial germ cells (PGCs) were detected by Oct4-immunostaining (arrowheads). At E13.5, mutant ovaries were devoid of germ cells detected by AP staining. Al: allantois; ne: neuroepithelium; he: hinggut epithelium; hg: hindgut; da: dorsal aorta; dm: dorsal mesentery; mn mesonephros; ov: ovary. Scale bars, 100 um. (B) Quantification of PGCs detected by AP-staining in different developmental stages. (C) Apoptosis (TUNEL assay) in E9.0 embryo sections of hindgut endoderm. SSEA1-expressing PGCs and apoptotic cells are marked by arrowheads and arrows, respectively. Note the apoptotic PGC in knockout section. Scale bar: 20 um.

Copyright

This image is from Pirouz M, PLoS Genet 2013 Aug;9(8):e1003712, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:223048

Associated Alleles

Symbol	Name
Mad2l2^tm1.1Mpz	MAD2 mitotic arrest deficient-like 2; targeted mutation 1.1, Mehdi Pirouz

Associated Genotypes

Allelic Composition	Genetic Background
Mad2l2^tm1.1Mpz/Mad2l2^tm1.1Mpz	involves: 129S2/SvPas * BALB/cJ