Phenotype Image Detail

Image

Caption

The compromised foot process interdigitation and aberrant distribution of the slit diaphragm components in Tjp1^tm1.1Whun/Tjp1^tm1.1Whun Tg(Nphs1-cre)33Mska/0 (Tjp1^deltapod) mice. (A-C) SEM analyses of glomeruli from the control and mutant mice. Foot processes were severely disorganized in the mutant mice at 6 weeks of age (A). The loss of interdigitation and the impaired adhesion to the glomerular basement membrane (GBM) of the podocyte foot processes was detected in the mutant mice at 2 weeks of age (B). At an earlier time point, 1 week of age, the foot processes were attached to the GBM but the interdigitation was disorganized in the mutant mice (C). Scale bars, 2 um (A, B, C top panels), 0.5 um (C bottom panels). (D) Immunofluorescence analyses of podocyte components: nephrin, podocin, Fyn, and synaptopodin (Synpo). The distribution of the slit diaphragm membrane proteins, nephrin and podocin, was fragmented and the signal intensity was reduced in the mutant mice at 1 week and 2 weeks of age. The signal of the actin-binding protein Synpo was increased in the mutant mice at 2 weeks of age. Fyn, a member of src family kinase, was not affected.

Copyright

This image is from Itoh M, PLoS One 2014;9(9):e106621, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:226210

Associated Alleles

Symbol	Name
Tg(Nphs1-cre)33Mska	transgene insertion 33, Taiji Matsusaka
Tjp1^tm1.1Whun	tight junction protein 1; targeted mutation 1.1, Walter Hunziker

Associated Genotypes

Allelic Composition	Genetic Background
Tjp1^tm1.1Whun/Tjp1^tm1.1Whun Tg(Nphs1-cre)33Mska/0	involves: C57BL/6 * C57BL/6N * DBA/2