Phenotype Image Detail

Image

Caption

Best1^tm1Web/Best1^tm1Web (KO) spermatozoa showing enhanced decapitation and angulated sperm tails. Percentages of straight tails and (E) stained (dead) and colorless (live) spermatozoa from the cauda epididymis after eosin-nigrosin staining from 100 spermatozoa of wild-type (WT) (n = 10) and mutant (n = 5) mice. (F) Mutant mice showing acrosome-reacted spermatozoa using PNA-488 staining. Percentages of acrosome intact cells (uniform apple green fluorescence) to acrosome-reacted sperm (perforated or absent acrosome cap) from 150 spermatozoa of WT (n = 16) and mutant mice (n = 10) of spermatozoa from caput, corpus, and cauda epididymis. (G) Box and Whisker plots of sperm velocity parameters including VSL, VCL, ALH, and VAP. Each box plot shows the interquartile range of 382 spermatozoa from CD-1 WT (n = 3) and 654 spermatozoa from mutant mice (n = 7). Spermatozoa were released into TYH290 media. Values are given as mean + SD. Two-sided Student's t test or Wilcoxon signed rank test: * P < 0.05; ** P < 0.01; *** P < 0.001.

Copyright

This image is from Milenkovic A, Proc Natl Acad Sci U S A 2015 May 19;112(20):E2630-9. Copyright 2015 National Academy of Sciences, U.S.A. J:221655

Associated Alleles

Symbol	Name
Best1^tm1Web	bestrophin 1; targeted mutation 1, Bernard HF Weber

Associated Genotypes

Allelic Composition	Genetic Background
Best1^tm1Web/Best1^tm1Web	involves: CD-1