Phenotype Image Detail

Image

Caption

Fen1^tm3.1Bhsh/Fen1^tm3.1Bhsh (ED) mice develop subcutaneous inflammation, lymphoproliferative disorder, and extramedular hematopoiesis. (a) Fen1^tm3.1Bhsh/ Fen1⁺ (ED/+) and Fen1^tm3.1Bhsh/Fen1^tm3.1Bhsh (ED/ED) mice older than 6 months developed subcutaneous nodule, which was characterized as epidermoid inclusion cysts with associated lympho-plasmacytic infiltrate. (b) The subcutaneous inflammation frequency of wild-type (WT) (n = 68), heterozygous (ED/+) (n = 74), and homozygous mutant (ED/ED) (n = 85) mice. *P = 0.0042 and **P < 0.0001 for comparison of the disease frequency in heterozygous and homozygous mutant mice with that in WT mice, respectively. (c)-(e) Mutant mice develop lymphoproliferative phenotype, characterized by marked follicular and interfollicular hyperplasia (d). Large cluster or sheet-like plasma cells, in 35% cases, occur in the interfollicular region. Panel e shows the lymphoproliferative disease frequency of WT (n = 16) and ED (n = 27) mice. *P < 0.0001 and **P = 0.014, (f)-(h) Homozygous mutant mice develop extramedular hematopoiesis. The mutant mice develop striking extramedullar hematopoiesis (EMH, g). The degree of EMH was arbitrarily defined as mild (+), moderate (++), and prominent (+++) based on the number and the distribution of megakaryocytes and erythroid precursor cells in spleen. Panel h shows the EMH frequency of WT (n = 16) and mutant (n = 34) mice. *not significant, **P = 0.006, and ***P = 0.05. All P values were calculated by Fisher Exact two-tail analysis. Scale bar, 100 um.

Copyright

This image is from Zheng L, Nat Med 2007 Jul;13(7):812-9 and is displayed with the permission of The Nature Publishing Group who owns the Copyright. J:231218

Associated Alleles

Symbol	Name
Fen1^tm3.1Bhsh	flap structure specific endonuclease 1; targeted mutation 3.1, Binghui Shen

Associated Genotypes

Allelic Composition	Genetic Background
Fen1^tm3.1Bhsh/Fen1^tm3.1Bhsh	Not Specified