Phenotype Image Detail

Image

Caption

Free spines and mismatched parallel fiber (PF)-purkinje cell (PC) synapses in Grid2^ts3/Grid2^ts3 cerebella. (A-E) Conventional electron microscopy in control (A, B) and mutant (C-E). A) Asterisks indicate PC spines contacting with PF terminals. B) Higher magnification image of a normal spine in WT. C) Letters f and m indicate free spines and mis-matched PF-PC synapses, respectively. High-power images of free spine (D) and mismatched synapse (E). Note that postsynaptic density (PSD), as indicated between two arrowheads, is well-matched with the synaptic terminal in normal control (B). In contrast, the free spine and mismatched synapse do not have any contact with PF synaptic terminal (D) or has a significantly longer PSD (E), respectively. Scale bars, 500 nm.

Copyright

This image is from Miyoshi Y, PLoS One 2014;9(9):e107867, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:222504

Associated Alleles

Symbol	Name
Grid2^ts3	glutamate receptor, ionotropic, delta 2; ts3

Associated Genotypes

Allelic Composition	Genetic Background
Grid2^ts3/Grid2^ts3	C57BL/6J-Grid2^ts3