Phenotype Image Detail

Image

Caption

Testes from 12-month-old Rnf8^{Gt(AS0574)Wtsi}/Rnf8^{Gt(AS0574)Wtsi} or Rnf8^{Gt(RRR260)Byg}/Rnf8^{Gt(RRR260)Byg} males (Rnf8-/-) exhibited a significant reduction in size compared with those of wild-type (WT) littermates. Bar, 0.25 cm. (D-K) Images of H&E-stained seminiferous tubules from 1.5-month-old WT (D and H) and homozygous mutant (E, F, I, and J) mice and a 3.5-month-old mutant male (G and K). H&E staining of testes from mutant males showed a spectrum of alterations in spermatogenesis. Mutant testis showed seminiferous tubules with well-populated layers of spermatogonia, spermatocytes, and mature spermatozoa (E and I) and other seminiferous tubules with only few or absent mature spermatozoa in the lumen and an unusually elevated number of immature spermatids and mitotic cells (E and I). Mutant testis showed disorganized epithelial architecture of seminiferous tubules, few spermatogonia and spermatocytes mixed together with apoptotic bodies, and the absence of mature sperm cells (F and J). Mutant testis showed focal vacuolar degeneration of most seminiferous tubules (G and K). In contrast to WT (L), H&E staining of epididymis of mutant mice (M, 20X) show no histological abnormalities; however, they have a significant reduction of mature sperms. Bars: (D-G) 100 um; (H-K) 50 um; (L and M) 100 um. A minimum of five mice per genotype have been analyzed.

Copyright

This image is from Li L, J Exp Med 2010 May 10;207(5):983-97, and is displayed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported License. J:161240

Associated Alleles

Symbol	Name
Rnf8^Gt(AS0574)Wtsi	ring finger protein 8; gene trap AS0574, Wellcome Trust Sanger Institute
Rnf8^Gt(RRR260)Byg	ring finger protein 8; gene trap RRR260, BayGenomics

Associated Genotypes

Allelic Composition	Genetic Background
Rnf8^Gt(RRR260)Byg/Rnf8^Gt(RRR260)Byg	involves: 129P2/OlaHsd * C57BL/6
Rnf8^Gt(AS0574)Wtsi/Rnf8^Gt(AS0574)Wtsi	involves: 129P2/OlaHsd * C57BL/6