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| Caption | Altered cell homeostasis in Neurog3-deficient small intestine. Sections of adult control (A, C, and E) and Neurog3tm1Fgu/Neurog3tm3.1Ggr Tg(Vil1-cre)20Syr/0 and Neurog3tm3.1Ggr/Neurog3tm3.1Ggr Tg(Vil1-cre)20Syr/0 (mutant) (B, D, and F) intestine were examined for the status of the proliferative crypt compartment (A, B, E, and F), villus length (measurements in G), and cell turn over (C, D, and measurement in H). (A and B) Immunofluorescence staining for the proliferative cell marker Ki67 clearly demonstrates an up to 2-fold enlargement of the proliferative crypt compartment (dashed bars in A and B) in mutant intestine. Arrows point to chromogranin A+ cells. (G) Measurement of the villi length indicates an approximately 40% reduction in their length in mutant intestine. (C and D) Twenty-four hours before dissection, adult control and mutant mice were injected with BrdU, and BrdU-labeled cells were then visualized by immunofluorescence staining. Then the distance from the villus base to last labeled BrdU+ cell was measured (dashed bars in C and D), demonstrating a 1.6-fold accelerated cell turnover in mutant intestine. Arrows point to chromogranin A+ cells. (E and F) H&E staining showing the enlargement of the crypt compartment seen in mutant intestine. n = 3. The age of the animals analyzed is 10-12 weeks. | ||||||||
| Copyright | This image is from Mellitzer G, J Clin Invest 2010 May 3;120(5):1708-21 and is displayed with the permission of the American Society for Clinical Investigation who owns the Copyright. J:161480 | ||||||||
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Associated Genotypes |
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
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last database update 10/29/2024 MGI 6.24 |
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