Phenotype Image Detail

Image

Caption

Impaired lipid absorption in Neurog3^tm1Fgu/Neurog3^tm3.1Ggr Tg(Vil1-cre)20Syr/0 and Neurog3^tm3.1Ggr/Neurog3^tm3.1Ggr Tg(Vil1-cre)20Syr/0 (mutant) mice. Oil red O, which stains neutral fats, was used to visualize lipid droplets in control (A) and mutant (B) tissue. Mutant small intestine clearly shows a strong reduction in the amount of lipid droplets (B) compared with control tissue (A). Arrowheads in B point to some lipid droplets found in mutant tissue. Ultrastructural analysis of the absorptive cells furthermore demonstrates a clear reduction in the number of neutral lipid-containing chylomicrons (arrows in C and D) in mutant intestine (D). (E and F) Ultrastructural analysis of exocrine pancreas at adult stage shows no difference in the number or quality of zymogen granules (arrowheads) in acinar cells in control (E) and mutant animals (F). The age of the mice analyzed is 10-12 weeks. Original magnification, 10,000 (C and D); 4,000 (E and F).

Copyright

This image is from Mellitzer G, J Clin Invest 2010 May 3;120(5):1708-21 and is displayed with the permission of the American Society for Clinical Investigation who owns the Copyright. J:161480

Associated Alleles

Symbol	Name
Neurog3^tm1Fgu	neurogenin 3; targeted mutation 1, Francois Guillemot
Neurog3^tm3.1Ggr	neurogenin 3; targeted mutation 3.1, Gerard Gradwohl
Tg(Vil1-cre)20Syr	transgene insertion 20, Sylvie Robine

Associated Genotypes

Allelic Composition	Genetic Background
Neurog3^tm1Fgu/Neurog3^tm3.1Ggr Tg(Vil1-cre)20Syr/0	involves: 129S2/SvPas * C57BL/6 * CD-1 * DBA/2 * SJL
Neurog3^tm3.1Ggr/Neurog3^tm3.1Ggr Tg(Vil1-cre)20Syr/0	involves: 129S2/SvPas * C57BL/6 * CD-1 * DBA/2 * SJL