Phenotype Image Detail

Image

Caption

Morphological defects and progressive hyperplasia in Prkar1a^tm1Lsk/Prkar1a^tm1Lsk Tg(Akr1b7-cre)1Anm/0 (AdKO) adrenals. (A-L) Adrenals were collected from wild-type (WT; A-C and G-I) and mutant (D-F and J-L) parous females aged of 5, 10 and 18 months and stained with haematoxylin and eosin. Mutant adrenal cortex presented progressive centrifugal expansion of large eosinophilic cells, indicated by a double arrow in (D-F). In the top panels, squares delineate the higher magnifications shown in (G-I) for WT and (J-L) for mutant adrenals. Insets, higher magnification illustrating the increased cell area of expanding eosinophilic cells compared to normal spongiocytes. (M-O) Adrenal sections from 18-month-old mice double-stained for beta-catenin, a zona glomerulosa marker (in green) and for Sf1, a steroidogenic marker (in red). Nuclei were stained by Hoechst in blue. (M) WT adrenal cortex. (N) Mutant adrenal cortex. (O) Higher magnification of (N), where hyperplastic small spindle-shaped, basophilic cells lacking both staining are enlightened by arrows. (P-R) Immunodetection of the pre-tumoural marker Gata-4 in adrenal sections of parous 18-month-old females. (P) WT. (Q-R) Mutant. (R) The square delineates the higher magnifications shown in the right. Top panel: Gata-4 staining. Lower panel: same section with haematoxylin and eosin counter-staining. M, Medulla; C, Cortex; F, zona fasciculata; G, zona glomerulosa; Ca, Capsule; Scale bars, 50 um.

Copyright

This image is from Sahut-Barnola I, PLoS Genet 2010;6(6):e1000980, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:161521

Associated Alleles

Symbol	Name
Prkar1a^tm1Lsk	protein kinase, cAMP dependent regulatory, type I, alpha; targeted mutation 1, Lawrence S Kirschner
Tg(Akr1b7-cre)1Anm	transgene insertion 1, Antoine Martinez

Associated Genotypes

Allelic Composition	Genetic Background
Prkar1a^tm1Lsk/Prkar1a^tm1Lsk Tg(Akr1b7-cre)1Anm/0	involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * DBA/2