Phenotype Image Detail

Image

Caption

Abnormal spermiogenesis in Pick1^tm1Rlh/Pick1^tm1Rlh (Pick1^-/-) mice. (A) H&E staining of the epididymis. The number of sperm in the homozygous mutant mice is lower than that in wild-type (WT) mice in the cross sections of the cauda epididymis. Insets: Enlarged views of the heads of the sperm, showing that the sperm from mutant mice are round-headed. Scale bars: 10 um. (B) The testes of mutant mice are smaller than those of WT or heterozygous mice. Scale bar: 2 mm. (C) Normalized testis weights (Testis weight (TW) 1000 / BW) of mutant mice are significantly lower than those of the WT or heterozygous mice. WT, 3.58 0.11; heterozygous, 3.25 0.09; homozygous, 2.88 0.19; n = 10. Data are presented as mean SEM; *P < 0.05, **P < 0.01. (D) The diameter of the seminiferous tubules in mutant mice is smaller than that of the WT and heterozygous mice. WT, 203.7 1.13 um; heterozygous, 206.2 5.2 um; homozygous, 181.3 4.2 um; n = 50. (E) H&E staining of testis. The lumens of seminiferous tubules in mutant mice are slightly larger than those in wild-type mice. The morphology of spermatogonia (white arrows), spermatocytes (white arrowheads), and round spermatids (black arrows) is similar in the 3 genotypes. However, there are fewer mature sperm in the mutant mice, and the heads of the sperm are round, in contrast to the hook-shaped heads found in WT and heterozygous mice (black arrowheads). The bottom row shows higher-magnification views of the boxed regions in the top panels. Scale bar: 10 um.

Copyright

This image is from Xiao N, J Clin Invest 2009 Apr;119(4):802-12 and is displayed with the permission of the American Society for Clinical Investigation who owns the Copyright. J:149846

Associated Alleles

Symbol	Name
Pick1^tm1Rlh	protein interacting with C kinase 1; targeted mutation 1, Richard L Huganir

Associated Genotypes

Allelic Composition	Genetic Background
Pick1^tm1Rlh/Pick1^tm1Rlh	involves: 129S1/Sv * 129X1/SvJ