Phenotype Image Detail

Image

Caption

Acrosome formation is disrupted in Pick1^tm1Rlh/Pick1^tm1Rlh (Pick1^-/-) mice. Immunostaining of testis sections. PICK1 (red) and acrosome (green) were labeled by a guinea pig antibody against PICK1 and a mouse antibody against sp56. Nuclei were marked by DAPI (blue). Four phases of spermiogenesis are shown in progressive order. (A) Images from wild-type (WT) mice. PICK1 signals (arrowheads) are close to the acrosomes (arrows) in the Golgi phase and the cap phase but move to opposite ends of the nucleus in the acrosome and maturation phases. Acrosomes grow from single granules in the Golgi phase to caps covering the heads of the nuclei in the cap phase to the crescent moon-shaped structures at one pole of the nuclei in the acrosome and maturation phases. (B) Images from knockout mice. PICK1 signals are notably missing in testes from homozygous mutant mice. There are multiple sp56-positive structures in spermatids of mutant mice throughout spermiogenesis. The morphology of the nuclei of the spermatids of WT and mutant mice was similar in the Golgi and cap phases. However, while the nuclei of WT mice become elongated in the acrosome and maturation phases, those of mutant mice remain round-shaped. Scale bars: 10 um. Right panels show higher-magnification views of the boxed regions in the left panels.

Copyright

This image is from Xiao N, J Clin Invest 2009 Apr;119(4):802-12 and is displayed with the permission of the American Society for Clinical Investigation who owns the Copyright. J:149846

Associated Alleles

Symbol	Name
Pick1^tm1Rlh	protein interacting with C kinase 1; targeted mutation 1, Richard L Huganir

Associated Genotypes

Allelic Composition	Genetic Background
Pick1^tm1Rlh/Pick1^tm1Rlh	involves: 129S1/Sv * 129X1/SvJ