Phenotype Image Detail

Image

Caption

Transmission electron microscopy (TEM) study of acrosome formation in Pick1^tm1Rlh/Pick1^tm1Rlh (Pick1^-/-) mice. TEM images of spermatids showing 4 phases of spermiogenesis. (A) In the wild-type (WT) mice, a large number of Golgi-derived proacrosomal granules are present in the Golgi phase, when a single acrosome granule can be seen attached to the nuclear envelope. The acrosome flattens and grows to form a cap at one end of the nucleus in the cap phase. The acrosome becomes extended along the nuclear envelope in the subsequent acrosome and maturation phases. The nucleus starts to elongate in the acrosome phase and becomes hook-shaped in the maturation phase. (B) In mutant mice, multiple acrosomal vesicular structures are seen in all phases of spermatids, as indicated by the arrows. The nuclei of spermatids of mutant mice failed to elongate and remain round in the maturation phase. Right panels show higher-magnification views of the boxed regions in the left panels. (C) Sperm from the caput epididymis of WT and mutant mice. While mitochondria wrap around the axoneme in the midpiece of sperm from WT mice, they aggregate around the deformed nucleus in mutant mice. The deformed acrosome is also shown. Scale bars: 1 um. ms, mitochondria sheath.

Copyright

This image is from Xiao N, J Clin Invest 2009 Apr;119(4):802-12 and is displayed with the permission of the American Society for Clinical Investigation who owns the Copyright. J:149846

Associated Alleles

Symbol	Name
Pick1^tm1Rlh	protein interacting with C kinase 1; targeted mutation 1, Richard L Huganir

Associated Genotypes

Allelic Composition	Genetic Background
Pick1^tm1Rlh/Pick1^tm1Rlh	involves: 129S1/Sv * 129X1/SvJ