Phenotype Image Detail

Image

Caption

Atg4b^{Gt(A029E06)Wrst}/Atg4b^{Gt(A029E06)Wrst} (Atg4B-/-) mice present an accumulation of abnormal globular substance in utricular/saccular lumen. (A) Representative semithin sections of wild-type (WT) and mutant mice utricles (left panels) showing the presence of otoconial crystals (arrows) and nascent globular substances (arrowheads) in WT samples. In contrast, mutant samples exhibited an accumulation of globular substances that usually presented a less dense content than those from WT mice as well as one or several internal vesicles (middle and left panels). (B) H&E sections from neonate mice utricles (left panels) showing the presence of normal otoconial crystals in WT samples (arrows) and an accumulation of eosinophilic globular substances in Atg5^tm1Nmz/Atg5^tm1Nmz neonates (arrowheads). At the ultrastructural level (right panels), Atg5 mutant globular substances presented similar features than those observed in Atg4 mutant mice. Scale bars: 15 um in semithin and H&E sections; 1 um in TEM panels.

Copyright

This image is from Marino G, J Clin Invest 2010 Jul 1;120(7):2331-44 and is displayed with the permission of the American Society for Clinical Investigation who owns the Copyright. J:163712

Associated Alleles

Symbol	Name
Atg4b^Gt(A029E06)Wrst	autophagy related 4B, cysteine peptidase; gene trap A029E06, German Gene Trap Consortium
Atg5^tm1Nmz	autophagy related 5; targeted mutation 1, Noboru Mizushima

Associated Genotypes

Allelic Composition	Genetic Background
Atg5^tm1Nmz/Atg5^tm1Nmz	involves: 129S1/Sv * 129X1/SvJ
Atg4b^Gt(A029E06)Wrst/Atg4b^Gt(A029E06)Wrst	involves: 129S2/SvPas * C57BL/6