Phenotype Image Detail

Image

Caption

BrdU-labeled neural crest cells in the embryonic anterior ocular segments. At E13.5, there were no differences found for the distribution and the number of Beta-gal-positive neural crest cells between the Ext1^tm1Yama/Ext1^tm1Yama H2az2^{Tg(Wnt1-cre)11Rth}/0 (KO) embryos (B and I) and control embryos (A and I). There was a significantly smaller number of BrdU-labeled cells in the central cornea (arrows) in the stroma of the Ext1^tm1Yama/Ext1^tm1Yama H2az2^{Tg(Wnt1-cre)11Rth}/0 embryos (D) as compared with the control embryos (C). Moreover, only a small number of BrdU-labeled cells were present in the corneal endothelium (arrowheads in E and F) at E15.5. (G and H). In mutant embryos at E15.5, BrdU-positive cells in the iridocorneal angle (open arrows) were also decreased. Original magnification, 20 (A-H). (J) The total number of BrdU-positive cells in the central cornea and iridocorneal angle in the mutant embryos was significantly smaller, as compared with the control eyes. Data represent mean SEM. *P < 0.01, Student's t test (n = 6).

Copyright

This image is from Iwao K, J Clin Invest 2009 Jul;119(7):1997-2008 and is displayed with the permission of the American Society for Clinical Investigation who owns the Copyright. J:152572

Associated Alleles

Symbol	Name
Ext1^tm1Yama	exostosin glycosyltransferase 1; targeted mutation 1, Yu Yamaguchi
H2az2^Tg(Wnt1-cre)11Rth	H2A.Z histone variant 2; transgene insertion 11, David H Rowitch

Associated Genotypes

Allelic Composition	Genetic Background
Ext1^tm1Yama/Ext1^tm1Yama H2az2^Tg(Wnt1-cre)11Rth/H2az2^+	B6.Cg-H2az2^Tg(Wnt1-cre)11Rth Ext1^tm1Yama