Phenotype Image Detail

Image

Caption

Abnormalities of Stac3^{tm1a(KOMP)Wtsi}/Stac3^{tm1a(KOMP)Wtsi} (KO) mice. (A) Complete paralysis, wrist drop, and abnormally rounded lunar appearance of a newborn mutant mouse. (B) Alveoli of mutant lungs are not inflated, as shown by Masson's trichrome staining. (C) Whole-mount E18.5 skeletons prepared with alcian blue, a cartilage stain, and alizarin red S, a bone stain, show numerous abnormalities in mutants, including abnormal spinal curvature, growth defects in costal cartilages, and decreased bone formation at muscle insertion sites. (D) H&E sagittal paraffin sections of E16.5 heads show decreased muscle mass of the tongue and abnormal clustering of nuclei in mutants. Mutant fibers also have fewer observable striations, and most have a vacuolated appearance. (E) Electron microscopy of E15.5 tongues shows that sarcomeres are present in mutant fibers as seen here but overall, sarcomeres appear more disorganized. (Scale bars, 1 um.)

Copyright

This image is from Nelson BR, Proc Natl Acad Sci U S A 2013 Jul 16;110(29):11881-6. Copyright 2013 National Academy of Sciences, U.S.A. J:222015

Associated Alleles

Symbol	Name
Stac3^tm1a(KOMP)Wtsi	SH3 and cysteine rich domain 3; targeted mutation 1a, Wellcome Trust Sanger Institute
Stac3^tm1a(KOMP)Wtsi	SH3 and cysteine rich domain 3; targeted mutation 1a, Wellcome Trust Sanger Institute

Associated Genotypes

Allelic Composition	Genetic Background
Stac3^tm1a(KOMP)Wtsi/Stac3^tm1a(KOMP)Wtsi	involves: C57BL/6N