Phenotype Image Detail

Image

Caption

Microvessel sprouting and microtube formation is impaired in P10 and adult Elk3^tm1.2Ics/Elk3^tm1.2Ics [Elk3(-/-)] knockout aortic ring explants. (A left) Aortic rings from wild-type (WT) animals show robust microvessel sprouting on day 5 of culture. In contrast, aortic rings from mutant knockout animals show drastically reduced microvessel sprouting. (A middle) WT, but not mutant, aortic ring endothelial cells form microtubes in Matrigel after three weeks of culture. (A right) Phalloidin staining of aortic ring endothelial cells cultivated in Matrigel shows interconnected endothelial cells (ECs) and tip cells with filopodia in control explants, whereas mutant aortic ring endothelial cells lack connections and instead have degenerated bulbs.

Copyright

This image is from Weinl C, PLoS One 2014;9(9):e107048, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:221521

Associated Alleles

Symbol	Name
Elk3^tm1.2Ics	ELK3, member of ETS oncogene family; targeted mutation 1.2, Mouse Clinical Institute

Associated Genotypes

Allelic Composition	Genetic Background
Elk3^tm1.2Ics/Elk3^tm1.2Ics	involves: 129S2/SvPas * C57BL/6J