Phenotype Image Detail

Image

Caption

Severe neuron loss in the motor cortex and the cerebellum of Spg11^{Gt(EUCE0085f05)Hmgu}/Spg11^{Gt(EUCE0085f05)Hmgu} (KO) mice. (A-B) Brain size is reduced in 16-month-old mutant compared to wild-type (WT) mice. Scale bars: 2 mm. (D-E) Neuron loss in the motor cortex of 16-month-old mutant mice. Hoechst-33258 (blue; nuclei), NeuN (red; neuronal marker), and Ctip2 (red; marker for layer V neurons) staining of the motor cortex at 16 months of age from WT (D) and mutant (E) mice. Individual cortical layers are labeled (I-VI). Scale bars: 100 um. (I-J) Semithin sections of the lumbar corticospinal tract show a loss of large diameter axons in 8- and 16-month-old mutant mice (n = 3; Student's t-test: *** indicates p<0.001). Scale bars: 20 um. (L-M) Purkinje-cell loss in aged mutant (M) but not WT (L) mice. ML: Molecular layer; PCL: Purkinje cell layer; GL: granular layer. The somata of Purkinje cells is indicated by a dotted line.

Copyright

This image is from Varga RE, PLoS Genet 2015 Aug;11(8):e1005454, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:228803

Associated Alleles

Symbol	Name
Spg11^Gt(EUCE0085f05)Hmgu	SPG11, spatacsin vesicle trafficking associated; gene trap EUCE0085f05, Helmholtz Zentrum Muenchen GmbH

Associated Genotypes

Allelic Composition	Genetic Background
Spg11^Gt(EUCE0085f05)Hmgu/Spg11^Gt(EUCE0085f05)Hmgu	involves: 129P2/OlaHsd * C57BL/6