Phenotype Image Detail

Image

Caption

Autofluorescence and glial fibrillary acidic protein (GFAP) immunoreactivity in different regions of the central nervous system in Spg11^{Gt(EUCE0085f05)Hmgu}/Spg11^{Gt(EUCE0085f05)Hmgu} (KO) mice. (A-D) At 16 months of age we observed an accumulation of autofluorescent material in cortical neurons of mutant mice (B). The neuron loss was paralleled by an activation of astrocytes in mutant mice as evident from GFAP stainings (D). Cortical layers are labeled (I-VI). (E-H) Accumulation of autofluorescent material was also obvious in the cerebellum of mutant mice (F). As in the cortex the neuron loss was paralleled by an activation of astrocytes in mutant mice (H). GL: granular cell layer, PKC: Purkinje cell layer, ML: molecular layer. (I-X) Though neurons in the hippocampus (I-J), vestibular nuclei (VN) (M,O), inferior olivary nucleus (ION) (N,P) and in the spinal cord (U,V) accumulate autofluorescent material, no activation of astroglia was observed for these regions (K,L,Q-T,W,X). Scale bars: 150 (A-H), 250 (I-L,U-X), and 50 um (M-T). CA1: Cornu ammonis 1. CA3: Cornu ammonis 3. DG: dentate gyrus. AF: autofluorescence.

Copyright

This image is from Varga RE, PLoS Genet 2015 Aug;11(8):e1005454, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:228803

Associated Alleles

Symbol	Name
Spg11^Gt(EUCE0085f05)Hmgu	SPG11, spatacsin vesicle trafficking associated; gene trap EUCE0085f05, Helmholtz Zentrum Muenchen GmbH

Associated Genotypes

Allelic Composition	Genetic Background
Spg11^Gt(EUCE0085f05)Hmgu/Spg11^Gt(EUCE0085f05)Hmgu	involves: 129P2/OlaHsd * C57BL/6