Phenotype Image Detail

Image

Caption

Purkinje cells in Spg11^{Gt(EUCE0085f05)Hmgu}/Spg11^{Gt(EUCE0085f05)Hmgu} (KO) mice accumulate abnormal autolysosomes. (A-B") While the small autofluorescent particles (AF) in wild-type (WT) Purkinje cells (A") are rarely associated with the lysosomal membrane protein Lamp1, autofluorescent structures in mutant Purkinje cells stain for Lamp1 at their periphery (B"). (C-D") The autofluorescent material in mutant Purkinje cells contains p62 (D") suggesting that the vesicular structures positive for both Lamp1 and p62 represent autolysosomes. Purkinje cell somata are marked by a dashed line. Age at sampling: 16 months. AF: autofluorescence; Scale bars: 10 um.

Copyright

This image is from Varga RE, PLoS Genet 2015 Aug;11(8):e1005454, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:228803

Associated Alleles

Symbol	Name
Spg11^Gt(EUCE0085f05)Hmgu	SPG11, spatacsin vesicle trafficking associated; gene trap EUCE0085f05, Helmholtz Zentrum Muenchen GmbH

Associated Genotypes

Allelic Composition	Genetic Background
Spg11^Gt(EUCE0085f05)Hmgu/Spg11^Gt(EUCE0085f05)Hmgu	involves: 129P2/OlaHsd * C57BL/6