Phenotype Image Detail

Image

Caption

Glomerulocystic phenotype in Arhgap35^m1Bchd/Arhgap35^m1Bchd (Arhgap35^D34/D34) is associated with a ciliogenesis defect. (A) Immunofluorescent staining of control and mutant glomeruli sections at E17.5 show that podocytes (marked by nephrin expression) form ZO-1+ tight junctions, reflective of an intact slit diaphragm (closed arrowheas). Scale bars, 10 um. (B-D) Immunofluorescent staining for acetylated alpha-tubulin and Arl13b in proximal tubules (marked by Lotus Tetragonolobus Lectin; LTL) (panel B) shows a significant decrease in the number (panel C) and length (panel D) of ciliated cells in mutant animals compared to wild-type (open arrowheads). Scale bars, 5 um. *p<0.05, **p<0.01, ***p<0.005 (unpaired, two-tailed Student's t-test).

Copyright

This image is from Stewart K, PLoS Genet 2016 Feb;12(2):e1005785, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:232342

Associated Alleles

Symbol	Name
Arhgap35^m1Bchd	Rho GTPase activating protein 35; mutation 1, Maxime Bouchard

Associated Genotypes

Allelic Composition	Genetic Background
Arhgap35^m1Bchd/Arhgap35^m1Bchd	involves: C3H/HeNCrl * C57BL/6J