Phenotype Image Detail

Image

Caption

At day 4 after tamoxifen induction, Myo5b^tm1.1Cle/Myo5b^tm1.1Cle Tg(Vil1-cre/ERT2)23Syr/0 (Myo5b^fl/fl;Vil-CreERT2) mice showed (C) subapical accumulation of PAS-positive vesicles (small arrows) and villus fusion (white arrowheads) and (D) mislocalization of the apical marker alkaline phosphatase (ALP) to the cytoplasm (arrows). (E and F) Transmission electron microscopy (TEM) revealed (E) erosion and loss of apical microvilli (large arrows), subapical accumulation of vesicles (small arrows), intracellular microvillus inclusions (black arrowhead), auto lysosomes (A/Ls), and widened intercellular spaces (*) in villus epithelial cells of Myo5b^tm1.1Cle/Myo5b^tm1.1Cle Tg(Vil1-cre/ERT2)23Syr/0 mice. (F) Electron microscopy (EM)-cytochemistry shows epithelial cells at the crypt-villus transition with PAS-positive, classical secretory granules (small arrows).

Copyright

This image is from Schneeberger K, Proc Natl Acad Sci U S A 2015 Oct 6;112(40):12408-13. Copyright 2015 National Academy of Sciences, U.S.A. J:227079

Associated Alleles

Symbol	Name
Myo5b^tm1.1Cle	myosin VB; targeted mutation 1.1, Hans Clevers
Tg(Vil1-cre/ERT2)23Syr	transgene insertion 23, Sylvie Robine

Associated Genotypes

Allelic Composition	Genetic Background
Myo5b^tm1.1Cle/Myo5b^tm1.1Cle Tg(Vil1-cre/ERT2)23Syr/0	involves: 129P2/OlaHsd * 129S4/SvJaeSor * C57BL/6 * DBA/2