Phenotype Image Detail

Image

Caption

Lower villus cells are the first cells affected by loss of MYO5B. Control and Myo5b^tm1.1Cle/Myo5b^tm1.1Cle Tg(Vil1-cre/ERT2)23Syr/0 mice were killed 2 days after tamoxifen injection. (A) PAS and (B) alkaline phosphatase (ALP) staining located subapically in epithelial cells at the crypt-villus transition and lower villus (arrows). (C) Transmission electron microscopy (TEM) confirmed subapical accumulation of vesicles (arrows) and widened intercellular space (*) in lower villus cells of mutant mice.

Copyright

This image is from Schneeberger K, Proc Natl Acad Sci U S A 2015 Oct 6;112(40):12408-13. Copyright 2015 National Academy of Sciences, U.S.A. J:227079

Associated Alleles

Symbol	Name
Myo5b^tm1.1Cle	myosin VB; targeted mutation 1.1, Hans Clevers
Tg(Vil1-cre/ERT2)23Syr	transgene insertion 23, Sylvie Robine

Associated Genotypes

Allelic Composition	Genetic Background
Myo5b^tm1.1Cle/Myo5b^tm1.1Cle Tg(Vil1-cre/ERT2)23Syr/0	involves: 129P2/OlaHsd * 129S4/SvJaeSor * C57BL/6 * DBA/2