Phenotype Image Detail

Image

Caption

Smad4 deficiency enhances TGFalpha-induced histological changes. The Smad4^tm1.1Gsu/Smad4^tm1.1Gsu Ptf1a^{tm1.1(cre)Cvw}/Ptf1a⁺ Tg(MtTGFA)Lmb/0 (STP) mice displayed similar but more pronounced architectural changes in the pancreata than the Tg(MtTGFA)Lmb/0 (MT-TGFalpha) mice, while no apparent change was detected in the pancreata of Smad4^tm1.1Gsu/Smad4^tm1.1Gsu Ptf1a^{tm1.1(cre)Cvw}/Ptf1a⁺ (S4) mice. The loss of Smad4 expression in the ductal epithelial cells was confirmed in the S4 and STP mice by immunohistochemistry (IHC). Histological sections from 8-months of zinc sulfate treated S4 (panels i, ii), MT-TGFalpha (panels iii, iv) and STP (panels v, vi) mice stained with H&E (panels i, iii, v); with antibodies to Smad4 (panels ii, iv, vi). Magnifications: panels 100x/200x; insets 400x.

Copyright

This image is from Garcia-Carracedo D, PLoS One 2015;10(3):e0120851, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:229184

Associated Alleles

Symbol	Name
Ptf1a^tm1.1(cre)Cvw	pancreas specific transcription factor, 1a; targeted mutation 1.1, Christopher VE Wright
Smad4^tm1.1Gsu	SMAD family member 4; targeted mutation 1.1, Gloria H Su
Tg(MtTGFA)Lmb	transgene insertion, Glenn Merlino

Associated Genotypes

Allelic Composition	Genetic Background
Smad4^tm1.1Gsu/Smad4^tm1.1Gsu Ptf1a^tm1.1(cre)Cvw/Ptf1a^+	involves: 129S1/Sv * 129X1/SvJ
Smad4^tm1.1Gsu/Smad4^tm1.1Gsu Ptf1a^tm1.1(cre)Cvw/Ptf1a^+ Tg(MtTGFA)Lmb/0	involves: 129S1/Sv * 129X1/SvJ * CD-1
Tg(MtTGFA)Lmb/0	involves: CD-1