Phenotype Image Detail

Image

Caption

Increased number of PanIN lesions in the Smad4^tm1.1Gsu/Smad4^tm1.1Gsu Ptf1a^{tm1.1(cre)Cvw}/Ptf1a⁺ Tg(MtTGFA)Lmb/0 (STP) mice compared to Tg(MtTGFA)Lmb/0 (MT-TGFalpha) mice. (A) Pancreas specimens from 4 and 8-months zinc sulfate treated MT-TGFalpha (panels i, iii, v, vii) and STP (panels ii, iv, vi, viii) mice were H&E stained (panels i-iv), co-immunolabeled (co-IHC) with antibodies against cytokeratin-19 (brown) and amylase (pink) (panels v, vi), or Alcian blue stained (panels vii, viii). MT-TGFalpha mice display ductal proliferation with increased acinar-to-ductal metaplasia (ADM) at both 4 and 8 months of treatment (panels i, iii). STP mice showed progression from ADM to PanIN-1 & -2 lesions (panels ii, iv). Arrows indicate ADM lesions and plus signs denote PanIN-1 lesions. The occurrence of ADMs in both models was demonstrated by co-IHC of Ck19 and amylase (panels v, vi). Alcian blue indicated vastly increased mucin contents within ducts in the STP mice (panel viii) over the MT-TGFalpha mice (panel vii). (B) Morphometric analysis of pancreata from MT-TGFalpha and STP mice treated with 8-months of zinc sulfate. Four mice per genotype were analyzed with a minimum of five fields counted per mouse (100x). (Upper panel): The graph depicts the average number of ducts per 200x field. There was no significant difference in the number of total ductal structures counted in both models. (Middle panel): The graph depicts the average number of PanIN-1 & -2 lesions per 200x field. The STP mice exhibited marked increase of PanIN-1 & -2 lesions than the MT-TGFalpha mice. (Lower panel): Percentage of Alcian blue positive area per 100x field was presented. The increased PanIN lesions were consistent to the enhanced Alcian blue positivity in the STP mice. Values are presented as mean SEM. (Student's t-test, ** p < 0.05). Magnifications: panels 100x; insets 400x.

Copyright

This image is from Garcia-Carracedo D, PLoS One 2015;10(3):e0120851, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:229184

Associated Alleles

Symbol	Name
Ptf1a^tm1.1(cre)Cvw	pancreas specific transcription factor, 1a; targeted mutation 1.1, Christopher VE Wright
Smad4^tm1.1Gsu	SMAD family member 4; targeted mutation 1.1, Gloria H Su
Tg(MtTGFA)Lmb	transgene insertion, Glenn Merlino

Associated Genotypes

Allelic Composition	Genetic Background
Smad4^tm1.1Gsu/Smad4^tm1.1Gsu Ptf1a^tm1.1(cre)Cvw/Ptf1a^+ Tg(MtTGFA)Lmb/0	involves: 129S1/Sv * 129X1/SvJ * CD-1
Tg(MtTGFA)Lmb/0	involves: CD-1