Phenotype Image Detail

Image

Caption

Bone tumorigenesis resulting from conditional heterozygous osteoblastic Prkar1a loss in Prkar1a^tm1.2Lsk/Prkar1a⁺ Tg(Col1a1-cre)1Kry/0 mice. (A) Full-body CT scan of a 33.6-week-old Prkar1a^tm1.2Lsk/Prkar1a⁺ Tg(Col1a1-cre)1Kry/0 mouse with bone tumors in the femur, spine, tail, and metatarsals (arrowheads). (D) H&E (left) and Masson's trichrome (right) staining of paraffin sections of a femur tumor from a 49.1-week-old Prkar1a^tm1.2Lsk/Prkar1a⁺ Tg(Col1a1-cre)1Kry/0 mouse. (F) TRAP histochemical stains of a 22.4-week-old Tg(Bglap2-TAg)1Rkho/0 (MOTO+) rib tumor (left), a 3.7-week-old Prkar1a^tm1.2Lsk/Prkar1a⁺ Tg(Col1a1-cre)1Kry/0 Tg(Bglap2-TAg)1Rkho/0 (Prkar1a^deltaOB/+/MOTO+) tibia tumor (middle), and 49.1-week-old Prkar1a^tm1.2Lsk/Prkar1a⁺ Tg(Col1a1-cre)1Kry/0 femur tumor (right) show abundant osteoclasts. Scale bars: 150 um (D); 50 um (D, insets, and F).

Copyright

This image is from Molyneux SD, J Clin Invest 2010 Sep 1;120(9):3310-25 and is displayed with the permission of the American Society for Clinical Investigation who owns the Copyright. J:165282

Associated Alleles

Symbol	Name
Prkar1a^tm1.2Lsk	protein kinase, cAMP dependent regulatory, type I, alpha; targeted mutation 1.2, Lawrence Kirschner
Tg(Col1a1-cre)1Kry	transgene insertion 1, Gerard Karsenty

Associated Genotypes

Allelic Composition	Genetic Background
Prkar1a^tm1.2Lsk/Prkar1a^+ Tg(Bglap2-TAg)1Rkho/0 Tg(Col1a1-cre)1Kry/0	involves: 129S1/Sv * 129X1/SvJ * FVB/N
Tg(Bglap2-TAg)1Rkho/0	FVB/N-Tg(Bglap2-TAg)1Rkho
Prkar1a^tm1.2Lsk/Prkar1a^+ Tg(Col1a1-cre)1Kry/0	involves: 129S1/Sv * 129X1/SvJ * FVB/N