Phenotype Image Detail

Image

Caption

Osteosarcoma (OSA) characterization in Tg(Bglap2-TAg)1Rkho/0 (MOTO) and Prkar1a^tm1.2Lsk/Prkar1a⁺ Tg(Col1a1-cre)1Kry/0 Tg(Bglap2-TAg)1Rkho/0 mice (Prkar1a^deltaOB/+/MOTO⁺) mice. (A-D) Representative H&E stained OSA variants: MOTO mice exhibit a spectrum of bone neoplasms that vary in malignant appearance and site. (A) Benign well differentiated cortical growth in distal tibia. (B) Parosteal osteosarcoma: extracortical with only focal attachment to the periosteum of the tibia and no evidence of invasion. (C) Classical osteoblastic osteosarcoma originating in the femoral medulla that invades and replaces bone marrow and deposits osteoid. (D) Poorly differentiated fibroblastic osteosarcoma in femur that has destroyed regular bone structure but still produces some osteoid. (E-H) PKA+ and PKA- mouse OSA subclasses can not be distinguished histologically. Shown are H&E stained osteoblastic (E, F) and fibroblastic (G, H) osteosarcoma variants from representative end-stage (>20 weeks of age) tumors in the PKA- (E, G) and PKA+ (F, H) subclasses. (I-L) H&E and (M-P) Masson's trichrome stained Prkar1a^tm1.2Lsk/Prkar1a⁺ Tg(Col1a1-cre)1Kry/0 Tg(Bglap2-TAg)1Rkho/0 tumors. Medullary tibia (I) humeral (L) and cortical femoral (K) tumors displaying the features of osteoblastic OSA with disorganized trabecular-like structures interspersed with neoplastic cells depositing osteoid. Tibial (N) and femural (O) tumors having cellular areas that are more fibroblastic in appearance but that still deposit osteoid as evidenced by pale green trichrome staining (N). (J) Tumor of periosteal origin invading into cortex of distal tibia. Tibial (M, O) and humoral (P) tumors with osteoid deposition. Mice are 3.4-4.1 weeks of age. (Q) Alkaline phosphatase immunostaining of Prkar1a^tm1.2Lsk/Prkar1a⁺ Tg(Col1a1-cre)1Kry/0 Tg(Bglap2-TAg)1Rkho/0 humerus tumor showing positive osteoblasts (red).

Copyright

This image is from Molyneux SD, J Clin Invest 2010 Sep 1;120(9):3310-25 and is displayed with the permission of the American Society for Clinical Investigation who owns the Copyright. J:165282

Associated Alleles

Symbol	Name
Prkar1a^tm1.2Lsk	protein kinase, cAMP dependent regulatory, type I, alpha; targeted mutation 1.2, Lawrence Kirschner
Tg(Bglap2-TAg)1Rkho	transgene insertion 1, Rama Khokha
Tg(Col1a1-cre)1Kry	transgene insertion 1, Gerard Karsenty

Associated Genotypes

Allelic Composition	Genetic Background
Prkar1a^tm1.2Lsk/Prkar1a^+ Tg(Bglap2-TAg)1Rkho/0 Tg(Col1a1-cre)1Kry/0	involves: 129S1/Sv * 129X1/SvJ * FVB/N
Tg(Bglap2-TAg)1Rkho/0	FVB/N-Tg(Bglap2-TAg)1Rkho