Phenotype Image Detail

Image

Caption

Melanoblast numbers are reduced in Rps7^Zma/Rps7⁺ Tg(Dct-lacZ)IPav/0 (Rps7^Zma/+; Tg(Dct-LacZ)) mutants. (A, B) In transgenic embryos carrying the melanoblast reporter Tg(Dct- LacZ), whole mount staining showed that Dct-positive melanoblasts are significantly reduced at E10.5 in Rps7^Zma/Rps7⁺ Tg(Dct-lacZ)IPav/0 mice (A) compared to Rps7⁺/Rps7⁺ Tg(Dct-lacZ)IPav/0 (Rps7+/+;Tg(Dct-LacZ)) littermates (B). The reduction is noticeably apparent over the otic region (red circle). (C,D) Whole mount staining of E14.5 mutant embryos showed that these embryos (D) also display a reduction in melanoblasts relative to WT littermates (C). The microphthalmia observed in heterozygous mutant mice is apparent in (D). (E, F) Consistent with the whole-mount observations, transverse vibratome sections through the trunk of E14.5 embryos revealed very few melanoblasts in the developing skin of mutant mice (F) as compared to the numerous melanoblasts seen in WT littermates (arrow, E). Blue punctate staining indicates positive signal in melanoblasts. In all pairs of images, WT and mutant are at the same magnification. NT = neural tube.

Copyright

This image is from Watkins-Chow DE, PLoS Genet 2013 Jan;9(1):e1003094, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:195156

Associated Alleles

Symbol	Name
Rps7^Zma	ribosomal protein S7; zuma
Tg(Dct-lacZ)IPav	transgene insertion I, William J Pavan

Associated Genotypes

Allelic Composition	Genetic Background
Rps7^Zma/Rps7^+ Tg(Dct-lacZ)IPav/0	involves: BALB/c * C3H/HeH * C57BL/6J * FVB/N