Phenotype Image Detail

Image

Caption

Decreased proliferation and increased apoptosis in Cul4b^tm1.1Yxg/Y (KO) embryos. (A) Paraffin sections of wild-type (WT) and mutant embryos at 7.5 dpc (days post coitum) were stained with an antibody against Ki67, a proliferation marker. Sections were counterstained with haematoxylin. Lower panels are the higher magnification of the upper panels. (B) Paraffin sections of WT and mutant embryos at 7.5 dpc were analyzed by immunostaining against BrdU, and counterstained with DAPI. Lower panels are the higher magnification of the upper panels. (C) Paraffin sections of wild-type and mutant embryos at 7.5 dpc were analysed by TUNEL assay for labeling apoptotic cells, and counterstained with DAPI. Lower panels are the higher magnification of the upper panels. (D) Paraffin sections of WT and mutant embryos at 7.5 dpc were stained with an antibody against cyclin E. Sections were counterstained with haematoxylin. Lower panels are the higher magnification of the upper panels.

Copyright

This image is from Jiang B, PLoS One 2012;7(5):e37070, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:199139

Associated Alleles

Symbol	Name
Cul4b^tm1.1Yxg	cullin 4B; targeted mutation 1, Yaoqin Gong

Associated Genotypes

Allelic Composition	Genetic Background
Cul4b^tm1.1Yxg/Y	involves: 129X1/SvJ * FVB/N