Phenotype Image Detail

Image

Caption

Plaque-associated neuroinflammation and abnormal neuronal architecture in Tg(tetO/Prnp-APP*Swe*Lon)9191Krz/0 Tg(Camk2a-tTA)1Mmay/0 (rTg9191, mutant) mice. The mutant mice show reactive gliosis in the vicinity of dense-core plaques. Brain sections from mutant mice at 24 months of age (B, E, H), their age-matched non-transgenic littermates (A, D, G), and age-matched Tg(APPSWE)2576Kha/0 (Tg2576) mice (C, F, I) were stained with antibodies directed against the astroglial marker S100Beta (A-C), a monoclonal antibody directed against the microglial marker ionized calcium binding adaptor molecule 1 (Iba1) (D-F), and an antibody directed against the astrocytic marker glial fibrillary acidic protein (GFAP) (G-I). Astrocytes and activated microglial cells reside near dense-core plaques visualized using Congo red (pink). Scale bar in I, 25 um, applies to A-I. (J-K) Mutant mice exhibited abnormal neuronal architecture around plaques. Thioflavin S (green) was used to visualize plaques and monoclonal antibody SMI-312 was used to visualize axons (red). (J) No plaques were detected in age-matched non-transgenic littermates of mutant mice, and neuronal morphologies were normal. (K) Plaques are surrounded by swollen, dystrophic axons (arrowheads) and curvy, distorted axonal processes (arrows) in brains of mutant mice. Scale bar in K, 50 um, applies to J and K. Representative photomicrographs show neuroinflammation and neuronal architecture of female mice, and similar results were found in male mice.

Copyright

This image is from Liu P, PLoS One 2015;10(5):e0126317, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:235351

Associated Alleles

Symbol	Name
Tg(Camk2a-tTA)1Mmay	transgene insertion 1, Mark Mayford
Tg(tetO/Prnp-APP*Swe*Lon)9191Krz	transgene insertion 9191, Kathleen R Zahs

Associated Genotypes

Allelic Composition	Genetic Background
Tg(Camk2a-tTA)1Mmay/0 Tg(tetO/Prnp-APP*Swe*Lon)9191Krz/0	involves: 129S6/SvEvTac * FVB/N