Phenotype Image Detail

Image

Caption

Representative bright filed images of TRAP-stained osteoclasts grown by co-culture of wild-type (WT) and Tmem178^tm1Lex/Tmem178^tm1Lex (Tmem178-/-) bone marrow macrophages (BMMs) and bone marrow stromal cells as indicated. Increased osteoclastogenesis is observed in the coculture system with bone marrow stromal cells. (B) Representative bright field images of resorptive pits in WT and mutant BMMs cultured on bovine bone slices in the presence of 10 ng/mL M-CSF and 50 ng/mL RANKL for 10 days. Resorption pits were visualized by staining with peroxidase-conjugated wheat-germ agglutinin. Bone resorption is increased in mutant cells cultured on bone for 10 days. Black lines delineate the resorbed areas. n = 8-9 per genotype.

Copyright

This image is from Decker CE, Proc Natl Acad Sci U S A 2015 Dec 22;112(51):15654-9. Copyright 2015 National Academy of Sciences, U.S.A. J:228311

Associated Alleles

Symbol	Name
Tmem178^tm1Lex	transmembrane protein 178; targeted mutation 1, Lexicon Pharmaceuticals
Tmem178^tm1Lex	transmembrane protein 178; targeted mutation 1, Lexicon Pharmaceuticals

Associated Genotypes

Allelic Composition	Genetic Background
Tmem178^tm1Lex/Tmem178^tm1Lex	involves: 129S5/SvEvBrd * C57BL/6