Phenotype Image Detail

Image

Caption

Abnormal atelectasis and hypercellularity phenotype in Fstl1^tm1.1Ning/Fstl1^tm1.1Ning mice. (A) Lung sections were prepared from the mutant mice at the indicated gestational stages and stained with H&E. No significant differences were detected between the wild-type (WT) and mutant embryos at E15.5 and E16.5, but dramatic differences were visible from E17.5 onward. A condensed appearance was observed in mutant lungs during this period. (Scale bars, 200 um.) (B) Cell proliferation by phosphohistone H3 (p-HH3) immunohistochemistry (IHC). Immunostaining of p-HH3 in WT and mutant lungs at E15.5 and E18.5, respectively. (Scale bars, 50 um.) (C) Cell proliferation was measured by BrdU labeling for lungs at E15.5 and E18.5. (Scale bars, 150 um.) (D) Quantification of cell proliferation by BrdU labeling in E15.5 WT and mutant lungs. The graph represents the mean of four independent experiments showing the comparison of the percentages of BrdU-positive cells in the epithelium and mesenchyme between WT and mutant lungs. (E) PAS staining showed dispersed cytoplasmic glycogen (pink stain) in mutant lungs. (Scale bars, 50 um.)

Copyright

This image is from Geng Y, Proc Natl Acad Sci U S A 2011 Apr 26;108(17):7058-63. Copyright 2011 National Academy of Sciences, U.S.A. J:171347

Associated Alleles

Symbol	Name
Fstl1^tm1.1Ning	follistatin-like 1; targeted mutation 1.1, Wen Ning

Associated Genotypes

Allelic Composition	Genetic Background
Fstl1^tm1.1Ning/Fstl1^tm1.1Ning	involves: 129S4/SvJae * C57BL/6J * FVB/N