Phenotype Image Detail

Image

Caption

Eye and lens phenotypes of heterozygous and homozygous Cryab^tm1.1Ady mice. Eyes were dilated in non-anesthetized mice and examined using a slit lamp. A) Slit lamp micrographs of 5-week-old mice. Left panel, wild-type (WT) mice with clear lenses. Middle panel, heterozygous mutant mice displaying low-grade opacities in the nuclear and posterior regions of the lens. Right panel, homozygous mutant mice with prominent opacity in the lens nucleus (stage 3) and the small eye phenotype. The dotted line shows the outline of the eye. B) Lens opacity and corneal abnormality in 10-month-old mutant mouse eyes. Top left, WT. Top right, left eye. Bottom left, right eye of a heterozygous mutant mouse. Bottom right, eye of homozygous mutant mouse showing complete (stage 4) opacity. C) Corneal abnormality in heterozygous mutant mouse eye. The WT eye was normal. E) Histological analysis of heterozygous mutant lenses. Lens sections were stained with hematoxylin and eosin (H&E). Top panel, WT lens with normal epithelial and fiber cell morphology. Middle panel, a heterozygous mutant lens with a multilayered region of cells. Lower panel, a higher magnification image of the multilayerd region shown in the rectangle of the middle panel. G) Immunofluorescence analysis of major intrinsic protein (MIP) expression in WT and heterozygous mutant lenses. G). Cross-sections of anterior cortical fibers of the lens. Sections of WT (left panel) and heterozygous (right panel) lenses are shown.

Copyright

This image is from Andley UP, PLoS One 2011;6(3):e17671, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:171679

Associated Alleles

Symbol	Name
Cryab^tm1.1Ady	crystallin, alpha B; targeted mutation 1.1, Usha P Andley

Associated Genotypes

Allelic Composition	Genetic Background
Cryab^tm1.1Ady/Cryab^tm1.1Ady	involves: 129X1/SvJ * C57BL/6 * C57BL/6J
Cryab^tm1.1Ady/Cryab^+	involves: 129X1/SvJ * C57BL/6 * C57BL/6J