Phenotype Image Detail

Image

Caption

E9.5 Axin2^canp/Axin2^canp homozygotes of the double tail class had conspicuous protrustions from the dorsal midline at different rostral-caudal positions: the fourth somite (A); the first somite (B); somite 14. (C and D) Expression of Brachyury (T) in e9.5 embryos. (C) T is expressed in the primitive streak and notochord of wild-type (WT) embryos. (D) The double tail embryo displayed an extra dorsal protrusion (arrow) that expressed T (in D and in Inset). The notochord expression of T was continuous in the anterior of the embryo, but broken in the posterior. (E-J) Histological analysis shows the nature of the neural tube defect in double tail embryos. Transverse sections of WT (E-G) and double tail mutants (canp-dt; H-J) at the level of the first brachial arch (E and H), heart (F and I), and the level of the 14th somite pair (G and J), where the protrusion arose. In contrast to the neural tube in WT embryos, double tail mutants had a flat neural plate at the branchial arch level and a collapsed neural plate at more posterior positions. A notochord was visible below the neural tube in the wild sections (arrows in E-G), but no notochord was detected in mutant sections at the level of the branchial arch and heart. In WT sections, somites flanked the neural tube. By contrast, somites were fused across the midline in double tail embryos. A transverse section through the protrusion showed that it arose from the middle of the neural plate and was composed of mesenchymal cells (arrow in J). (Scale bars, 50 um in E-J.)

Copyright

This image is from Qian L, Proc Natl Acad Sci U S A 2011 May 24;108(21):8692-7. Copyright 2011 National Academy of Sciences, U.S.A. J:171894

Associated Alleles

Symbol	Name
Axin2^canp	axin 2; canopus

Associated Genotypes

Allelic Composition	Genetic Background
Axin2^canp/Axin2^canp	C3.B6-Axin2^canp